Screening natural content of water soluble b vitamins in fish: enzymatic extraction, HILIC separation and tandem mass spectrometric determination

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dc.contributor.authorChatterjee, N.S.
dc.contributor.authorAshok Kumar, K.
dc.contributor.authorAjeeshkumar, K.K.
dc.contributor.authorRemya Kumari, K.R.
dc.contributor.authorVishnu, K.V.
dc.contributor.authorAnandan, R.
dc.contributor.authorSuseela Mathew
dc.contributor.authorRavishankar, C.N.
dc.date.accessioned2018-01-11T09:01:47Z
dc.date.available2018-01-11T09:01:47Z
dc.date.issued2017
dc.description.abstractDespite the potential of LC with tandem MS (MS/MS) in improving sensitivity and selectivity, analytical methods are scarce for the determination of protein-bound and phosphorylated forms of B vitamins in food. This prompted us to develop a method for LC-MS/MS determination of naturally occurring nicotinamide, nicotinic acid, thiamine, pyridoxine, riboflavin, pantothenic acid, biotin, folic acid, and cyanocobalamin in fish. Baseline separation of the vitamins was achieved in a hydrophilic interaction LC condition. An ultrasonication-assisted enzymatic extraction protocol for sample preparation was optimized and validated. The time required for extraction was significantly reduced (to 4 h), while maintaining good extraction efficiency. Acetonitrile content (80%, v/v) in the prepared sample was found to be optimum for excellent peak shape and sensitivity. The dynamic linear range of the vitamins ranged from 2.5 to 500 ng/g, and the regression coefficient values were greater than 0.99. LOQ values ranged from 0.4 to 50 ng/g for the different vitamins. The spike recovery values at 50 and 100 ng/g ranged from 87.5 to 97.5%. The intra- and interday precision values were satisfactory. Accuracy of the developed method was determined by analysis of a Certified Reference Material. The method could also be used for unambiguous determination of the natural content of the target vitamins in fish.en_US
dc.identifier.citationJ. AOAC Int. 100(3): 579-585en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3340
dc.language.isoenen_US
dc.titleScreening natural content of water soluble b vitamins in fish: enzymatic extraction, HILIC separation and tandem mass spectrometric determinationen_US
dc.typeArticleen_US
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