Sardine oil loaded vanillic acid grafted chitosan microparticles, a new functional food ingredient: attenuates myocardial oxidative stress and apoptosis in cardiomyoblast cell lines (H9c2)

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dc.contributor.authorVishnu, K.V.
dc.contributor.authorAjeeshkumar, K.K.
dc.contributor.authorChatterjee, N.S.
dc.contributor.authorLekshmi, R.G.K.
dc.contributor.authorSreerekha, P.R.
dc.contributor.authorMathew, S.
dc.contributor.authorRavishankar, C.N.
dc.date.accessioned2018-04-04T05:02:26Z
dc.date.available2018-04-04T05:02:26Z
dc.date.issued2017
dc.description.abstractFish oil has been widely recognized as an excellent dietary source of polyunsaturated n-3 fatty acids such as EPA and DHA. However, it can undergo oxidation easily resulting in the formation of toxic off flavor compounds such as hydro- peroxides. These compounds adversely affect the nutritional quality and may induce several stress reactions in body. To solve this problem, a new antioxidant bio-material, vanillic acid-grafted chitosan (Va-g-Ch), was synthesized and used as a wall material for microencapsulation of fish oil. The sar- dine oil loaded Va-g-Ch microparticles could be a potential functional food ingredient considering the numerous health benefits of fish oil, chitosan, and vanillic acid. The current study aimed to investigate the possible protective effect of sardine oil-loaded Va-g-Ch microparticles against doxorubicin-induced cardiotoxicity and the underlying mech- anisms. In vitro cytotoxicity evaluation was conducted using H9c2 cardiomyocytes. MTT assay revealed that effective cytoprotective effect was induced by a sample concentration of 12.5 μg/mL. Results of apoptosis by double fluorescent staining with acridine orange/ethidium bromide and caspase- 3 evaluation by ELISA substantiated the above findings. Further, flow cytometric determination of membrane poten- tial, relative expression of NF-κB by PCR, and ROS determi- nation using DCFH-DA also confirmed the protective effect of encapsulated sardine oil against doxorubicin-induced cardiotoxicity. NF-κB expression was down-regulated nearly by 50% on cells treated with encapsulated sardine oil. Altogether, the results revealed that sardine oil-loaded Va-g- Ch microparticles demonstrated potential cell protection against doxorubicin-induced oxidative stressen_US
dc.identifier.citationCell Stress Chaperones doi:10.1007/s12192-017-0834-5en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3742
dc.language.isoenen_US
dc.titleSardine oil loaded vanillic acid grafted chitosan microparticles, a new functional food ingredient: attenuates myocardial oxidative stress and apoptosis in cardiomyoblast cell lines (H9c2)en_US
dc.typeArticleen_US
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