Phenotypic and genotypic characterization of nonO1/ nonO139 Vibrio cholerae from aquaculture farm sediments
Phenotypic and genotypic characterization of nonO1/ nonO139 Vibrio cholerae from aquaculture farm sediments
Date
2020
Authors
Reethy, P. S.
Lalitha, K. V.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)
Abstract
Toxigenic Vibrio cholerae is a major public health
problem in developing countries andlysogenic
conversion of naturally occurring nontoxigenic V.
cholerae strains by a lysogenic filamentous bacteriophage
CTXÖ has also been demonstrated. The
aim of this study was to analyse phenotypic and
genotypic characteristics of V. cholerae strains from
sediments in aquaculture settings. Based on biochemical,
molecular and serological assays confirmed
the presence of fourty nine nonO1/nonO139
V.cholerae strains from fifty six sediment samples.
PCR assays revealed that all V.cholerae strains
harboured toxR, hlyA and ompW genes. But none of
the virulent genessuch as ctxA, tcpA, ace and zot were
present in all tested strains. Antibiotic susceptibility
to different antibacterial agents was also performed
by disc diffusion assay. This result has shown 26.5%
of the non-O1/non-O139 V.cholerae isolates were
multidrug resistant for three classes of antibiotics.
Genetic diversity of nontoxigenic V.cholerae isolated
from aquaculture farm sedimentswas determined by
ERIC PCR.The genetic diversity studies revealed
clonal relationship among the various non-O1/non-
O139 isolates.
Description
Keywords
Non-O1/Non-O139Vibrio cholerae, Aquaculture Farm Sediments, Multidrug resistance, ERICPCR