Functional characterization, secondary structure prediction and analysis of ectoine biosynthesis genes from Bacillus halodurans: An osmolyte involved in stress tolerance
Functional characterization, secondary structure prediction and analysis of ectoine biosynthesis genes from Bacillus halodurans: An osmolyte involved in stress tolerance
Date
2010
Authors
Rajan, L.A.
Joseph, T.C.
Thampuran, N.
James, R.
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
An open reading frame encoding the ectoine
biosynthesis genes was cloned from the Bacillus halodurans
genome. An expression plasmid containing the operon
was introduced into Escherichia coli cells, and the recombinant
ectoine was quantified. The secondary structure of
ectoine biosynthesis proteins were predicted and was quite
similar to that of reported proteins from eubacteria
Description
Keywords
Bacillus halodurans, compatible solutes, ectoine genes, osmoregulation, salt stress
Citation
World Journal of Microbiol Biotechnology 2010: DOI 10.1007/s11274-010-0379-6