Comparison of collagen extracted from skin of double spotted queenfish and Malabar grouper
Comparison of collagen extracted from skin of double spotted queenfish and Malabar grouper
Date
2014
Authors
Hema, G.S.
Shyni, K.
Mathew, S.
Ninan, G.
Joshy, C.G.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India), Cochin, India
Abstract
Acid soluble collagen (ASC) and pepsin digestible
collagen (PDC) from the skin of double-spotted
queenfish (Scomberoides lysan) and Malabar grouper
(Epinephelus malabaricus), were isolated and characterized.
On wet weight basis, the yields of ASC and
PDC from queen fish and grouper were 7.82, 3.92,
12.5 and 6.49% respectively. Amino acid analysis
revealed that they contained glycine as a major
amino acid with high contents of alanine, proline
and hydroxyproline. Based on sodium dodecyl
sulfate polyacrylamide gel electrophoretic patterns
and subunit compositions, all were identified to be
type 1 collagens when compared with calf skin type
1 collagen. a1, a2 and b chains were the major
components of the presently isolated collagens.
While comparing these two species, queen fish skin
had good yield of collagen which could be served
as an alternative source of collagen for different
applications.
Description
Keywords
acid soluble collagen, pepsin digestible collagen, yield, type 1 collagen
Citation
Fishery Technology 2014: 51(2), 93-97