Synthesis and biochemical characterization of silver nanoparticles grafted chitosan (Chi‑Ag‑NPs): in vitro studies on antioxidant and antibacterial applications
Synthesis and biochemical characterization of silver nanoparticles grafted chitosan (Chi‑Ag‑NPs): in vitro studies on antioxidant and antibacterial applications
Date
2020
Authors
Dara, Pavan Kumar
Mahadevan, R.
Digita, P. A.
Visnuvinayagam, S.
. Kumar, Lekshmi R. G
Mathew, Suseela
Ravishankar, C. N.
Anandan, R.
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Metal-based nanoparticles with potential antioxidant and antimicrobial properties have led to their incorporation of
biomaterials for human health care importance. The present investigation is to study on the silver nanoparticles grafted
chitosan (Chi-Ag-NPs) composite materials due to their potential applications. Chi-Ag-NPs were synthesized by chemical
reduction of silver nitrate salts and biodegradable chitosan. The obtained Chi-Ag-NPs were characterised for Fouriertransform
infrared spectroscopy (FTIR), scanning electron microscope (SEM), Transmission electron microscopy (TEM),
X-ray powder diffraction, antioxidant and antibacterial properties. UV–visible spectra showed the configuration proficiency
of Ag-NPs and chitosan. FTIR spectra indicated that the functional groups of chitosan have particular interactions
with Ag-NPs. Particle size and TEM analyses showed that Chi-Ag-NPs were equivalently dispersed in matrix with an
average size of 10–230 nm. The surface morphological structures of chitosan has played important role in influencing
the adsorption of Ag-NPs. Chi-Ag-NPs were tested for their bioactive properties and results exhibited higher antioxidant
and antibacterial activities by the addition of Ag-NPs. Chi-Ag-NPs composites were non-toxic to L929 fibroblast
cells. The present investigation suggested that chitosan will be a good source for the preparation of bionanocomposite
materials and also could be useful in different industrial and biomedical applications.
Description
Keywords
Chitosan silver nanoparticles, Antioxidant and antimicrobial properties