Distribution and genotypic characterization of salmonella serovars isolated from tropical seafood of cochin, india

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dc.contributor.authorKumar, R.
dc.contributor.authorSurendran, P.K.
dc.contributor.authorThampuran, N.
dc.date.accessioned2013-12-30T09:53:22Z
dc.date.available2013-12-30T09:53:22Z
dc.date.issued2009
dc.description.abstractAbstractAims:To determine the distribution of Salmonella serovars in seafood and to examine the intraserovar genetic variations in Salmonella enterica subsp. enterica serovar Rissen and Salmonella Weltevreden by polymerase chain reaction (PCR)-ribotyping and enterobacterial repetitive intergenic consensus (ERIC)-PCR methods.Method and Results:A total of 417 seafood samples collected over 2003-2006 from fishing harbours and fish markets of Cochin (India) was studied for presence of Salmonella serovars. Seafood samples were analysed for the presence of Salmonella by Bacteriological Analytical Manual (BAM), U.S. Food & Drug Administration (USFDA) method. The study indicated that 23.2% of the seafood samples were positive for Salmonella and a total of 241 Salmonella isolates comprising of 27 different serovars were isolated from seafood. S. Weltevreden, Salmonella Rissen, Salmonella Typhimurium and Salmonella Derby were found to be the most predominant serovars in seafood. PCR-ribotypes and ERIC-PCR profiles showed multiple genotypic profiles for S. Rissen and S. Weltevreden in seafood and the level of discrimination indices obtained was at 0.974 for S. Rissen and 0.988 for S. Weltevreden, respectively.Conclusion:The study highlighted the major Salmonella serovars in the seafood of Cochin (India) and molecular fingerprinting pattern revealed genetic variation among S. Rissen and S. Weltevreden.Significance and Impact of the Study:Widespread occurrence of Salmonella contamination in seafood and multiple clones of S. Rissen and S. Weltevreden detected in seafood, thus, indicated the diverse routes of Salmonella contamination in seafood.en_US
dc.identifier.citationJournal of Applied Microbiology 2009: 106(2), 515-524en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1154
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.subjectGenotypingen_US
dc.subjectribotypingen_US
dc.subjectsalmonellaen_US
dc.subjectseafooden_US
dc.titleDistribution and genotypic characterization of salmonella serovars isolated from tropical seafood of cochin, indiaen_US
dc.typeArticleen_US
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