Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish (Pangasius hypophthalmus)

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dc.contributor.authorK Vijayan, Divya
dc.contributor.authorPR, Sreerekha
dc.contributor.authorCS, Tejpal
dc.contributor.authorKK, Asha
dc.contributor.authorS, Mathew
dc.contributor.authorCN, Ravishankar
dc.contributor.authorR, Anandan
dc.date.accessioned2022-04-06T06:12:37Z
dc.date.available2022-04-06T06:12:37Z
dc.date.issued2018
dc.description.abstractThe aim of the present study was to extract high pure acid soluble collagen (ASC) from the airbladder of striped cat fish (Pangasius hypophthalmus). ASC was extracted and its physico-chemical characterization was carried out to confirm the purity and structural integrity of extracted collagen. The yield of ASC extract was determined to be 73.4±0.8% of the dry weight of raw material. Amino acid analysis revealed the glycine content as 243.0±1.3 residues/1000 residues, imino acids as 186.1±1.4 residues/1000 residues respectively and was shown to be devoid of non-collagenous amino acids such as tryptophan. Electrophoretic analysis confirmed the subunit pattern of type I collagen and showed that it also contained β and ɤ subunits. UV/Vis absorption and FT-IR analysis demonstrated the primary and secondary structural integrity of collagen and also indicated that no denaturation had occurred during extraction process. 1H-NMR analysis validated the structural integrity of collagen triple helix. The denaturation temperature (Td) of ASC was assessed as 33.3 °C. The air bladder collagen extracted from striped cat fish is of high purity and belongs to type I with intact triple helical structure.en_US
dc.identifier.citationInternational Journal of Fisheries and Aquatic Studies 6(4): 310-318en_US
dc.identifier.urihttp://drs.cift.res.in/handle/123456789/6007
dc.language.isoenen_US
dc.publisherInternational Journal of Fisheries and Aquatic Studiesen_US
dc.subjectCollagenen_US
dc.subjectair bladderen_US
dc.subjectstriped cat fishen_US
dc.subjectPangasius hypophthalmusen_US
dc.titleExtraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish (Pangasius hypophthalmus)en_US
dc.typeArticleen_US
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