Peer Reviewed Journal Articles (National) (M,F&B)

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Browsing Peer Reviewed Journal Articles (National) (M,F&B) by Author "Basha, K.A."

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    Effect of different organic acids on survival of larvae and control of water microflora in milkfish (Chanos chanos) hatchery system
    (Society of Fisheries Technologists (India), Cochin, 2018) Kumar, N.R.; Kumar, P.P.; Siddaiah, G.M.; Murugadas, V.; Basha, K.A.; Sivaraman, G.K.; Prasad, M.M.
    This study reports the use of organic acids (acetic, malic, formic and citric) in fish larval rearing systems for survival and controlling the microflora of rearing water. The milk fish (Chanos chanos) larvae was treated for 10, 15, 30 and 60 min with seven concentrations (%) viz., 1, 0.2, 0.1, 0.075, 0.05, 0.025 and 0.02 of organic acids respectively. The percentage survival was zero (1, 0.2, and 0.1% conc) for all the organic acid at various time periods. The effective concentrations for treating fish larvae are 0.05, 0.05, 0.025 and 0.075% concentrations of acetic, malic, formic and citric acid, respectively where 100% survival was observed. Similarly, when the larval rearing water was treated with different concentrations of same organic acids and time period, complete inhibition of microbial flora was observed (1, 0.2 and 0.1% conc) for all organic acids at various time periods. However, at concentrations less than 0.1%, total bacterial count (TBC) and presumptive Vibrio count (PVC) were found in the range of 3.19 to 5.75 and 2.22 to 3.58 log 10 cfu ml-1. In contrast, TBC and PVC in control group were found in the range of 6.37 to 6.90 and 4.35 to 4.74 log 10 cfu ml-1. The acid treatment with concentrations of 0.075, 0.05 and 0.025 except formic acid at 0.025, 0.020 significantly (p<0.05) improved the survival rates of fish larvae and reduced both TBC and PVC in larval rearing water. The present findings strongly recommend the use of organic acids for treating fish larvae for improved survival and also for reducing pathogenic bacterial load from larval rearing waters.
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    Need for an optimized protocol for screening seafood and aquatic environment for Shigella sp
    (Society of Fisheries Technologists (India), Cochin, 2019) Nadella, R.K.; Murugadas, V.; Joseph, T.C.; Lalitha, K.V.; Basha, K.A.; Muthulakshmi, T.; Prasad, M.M.
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    Prevalence, molecular characterization, genetic heterogeneity and antimicrobial resistance of Listeria monocytogenes associated with fish and fishery environment in Kerala, India
    (Society for Applied Microbiology, 2019) Basha, K.A.; Kumar, N.R.; Das, V.; Reshmi, K.; Rao, B.M.; Lalitha, K.V.; Joseph, T.C.
    The prevalence of Listeria monocytogenes in the retail fish markets of the Kerala, India was investigated by screening 227 samples comprising of marine finfish (n = 97) shellfish (n = 19), ready-to-cook fish products (n = 47), ready-to-eat fish products (n = 10), dried fish (n = 11) and retail ice (n = 43). The prevalence of L. monocytogenes and L. innocua was 2 7% and 17 2% respectively. Sample category wise, prevalence of L. monocytogenes was higher in marine finfish (1 8%) and retail ice (0 9%). All the L. monocytogenes isolates carried virulent genes namely inlA, inlC, inlJ, hlyA, iap, plcA, prfA genes and majority (82%) belonged to 1/2a, 3a serogroups. L. monocytogenes isolates were multidrug-resistant and showed resistance to ampicillin, penicillin, erythromycin, tetracycline and clindamycin. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) delineated 58% genetic heterogeneity among the L. monocytogenes strains. The study reports that genetic similarities of the isolates were interlinked to their serogroup and sample origin.