1999

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Browsing 1999 by Author "Dayal, R"

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    Cryopreservation of Brown Trout (Saltno trutta fario) Sperm: The Influence of Extender Composition and Fertilization Procedure
    (Society of Fisheries Technologists (India)Cochin, 1999) Gopalakrishnan, A; Thakur, K.L; Ponniah, A.G; Kumar, K; Dayal, R
    Seven extenders were tested to check their relative efficiency in cryopreserving the milt of brown trout (Saltno trutta fario). Hatching rates equal to that of control (p > 0.05) were obtained when a cryodiluent containing egg yolk (2%) and dimethylsulphoxide (10%) was used. Removal of coelomic fluid prior to the addition of thawed milt and addition of 5 mM theophylline to the "Dexter" solution enhanced the hatching percentage. The sperm density varied from 9.83 to 18.41 x 109 sperms/ml.