2021

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Browsing 2021 by Subject "DNA restriction"

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    Characterization of tailed phages against E. coli using DNA restriction digestion analysis
    (Society of Fisheries Technologists (India), 2021) Karthika, R.; George, Iris; Murugadas, V.; Jeswin, J.; Sherin, P. S. Anna; Sanjeev, Devi; Visnuvinayagam, S.; Manikanta, B.; Prasad, M. M.; Rao, B. Madhusudana
    In the context of emerging newer forms of antimicrobial resistance multidrug resistance (MDR), extensively drug resistance (XDR) and pan drug resistance (PDR) in pathogens, bacteriophages remain a promising alternative antimicrobial weapon to control it (Falagas & Karageorgopoulos, 2008, Magiorakos et al., 2012, Ghajavand et al., 2017). Bacteriophages are viruses that infect and kill bacteria and have many advantages when used alone or in combination with antibiotics viz., host specificity, self-limiting replications, harmlessness to the native microflora, longer period for resistance development, are significantly safer and are better tolerated without infecting mammalian cells (Principi et al., 2019). Bacteriophages can disperse a bacterial biofilm, which is very difficult to eradicate with standard antibiotic therapy (Lu & Collins, 2007). Bacteriophages enter the host bacteria through the receptors, replicate inside the host with the host cellular machinery and enter either lytic or lysogenic mode (Ofir & Sorek, 2018). Owing to these unique characteristics, bacteriophages are being freshly evaluated taxonomically (Sharma et al., 2017). Various aquatic environments such as sewage, canals, rivers, lakes, mangroves, coastal waters, aquaculture ponds, etc. remain a sink for the presence of diverse bacteriophages with variations in the host range of activities (Flu & Flu, 1947; Jin et al., 2019; Topka et al., 2019).