Peer Reviewed Journal Articles (National) (M,F&B)

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Browsing Peer Reviewed Journal Articles (National) (M,F&B) by Subject "E.coli"

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    Antibacterial activity of shrimp chitosan against Escherichia coli, Salmonella, and Pseudomonas aeruginosa isolated from seafoods
    (Society of Fisheries Technologists(India), Cochin, 2006) Kumar, R.; Surendran, P.K.; Thankappan, T.K.
    Antibacterial activity of shrimp shell based chitosan was determined against three food borne pathogens viz., Escherichia coli, Salmonella, and Pseudomonas aeruginosa isolated from seafoods. Tests were carried out in phosphate buffered saline (PBS) at pH 6.0. Four different concentrations, 0.01%, 0.03%, 0.05% and 0.1% (w/v) of chitosan were used against E.coli and Salmonella, and 0.01%, 0.05%, 0.5% and 1% were against P.aeruginosa. Chitosan showed stronger bactericidal activity against Salmonella compared to E.coli and P.aeruginosa as evidenced by 3 log cfu.ml super(-1) reduction in Salmonella count with 0.01% chitosan concentration. There was complete elimination of 5 log cfu.ml super(-1) cell count of Salmonella within 24 h at 0.1% chitosan level. There was 3 log reduction in E.coli count at 0.01% chitosan, but not completely inhibited even at the maximum used concentration (0.1%). The inhibition concentration against P.aeruginosa was higher level compared to Enterobacteriaceae and it was found that reduction in 5 log cfu.ml super(-1) was achieved only at 72 h of chitosan exposure. Different chitosan concentrations 0.05%, 0.1% and 1% had almost same inhibition pattern for P.aeruginosa at 6h. 18h 36h and 72h of exposure. The effectiveness of chitosan against tested pathogens showed Salmonella was most susceptible towards chitosan followed by E.coli and P.aeruginosa.
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    Assessment of microbial quality of fish processing industrial effluent in bar-mouth at Bhidia landing site, Veraval, Gujarat, India
    (Triveni Enterprises, 2016) Sivaraman, G.K.; Visnuvinayagam, S.; Jha, A.K.; Renuka, V.; Remya, S.; Deesha, V.
    The present study was carried out to assess the microbial quality of fish processing industries effluent at Bhidia bar- mouth, Veraval, Gujarat during April, 2012 to March 2013. The total viable bacterial count (TVBC), total Enterobacteriaceae count, E.coli count (EC), Staphylococcus aureus and Fecal Streptococcal count in effluent ranged from 3.0 x 10 to 6.8 x 10 , 9.0 x 10 to 2.9 x 10 , 0 to 0. 5 x 10 , 0 to 0. 4 x 10 and 0.3 x 10 to 0. 1 x 10 cfu.ml , respectively. Significantly higher load of TEC ,E.coli, S.aureus , Fecal Streptococci, Total coliforms and Fecal coliforms were higher during summer whereas, TVBC was higher in the month of Sept.-Oct. Furthermore, the total coliform and fecal coliform counts were found to be higher with 1400+ /100ml MPN value throughout the year of the study, except in the month of August. Overall occurrence of pathogenic strains of E.coli, S.aureus and Fecal streptococci were 41.67%, 25.00% and 66.67% respectively during this period. The antibiogram of the isolated E.coli isolates show that almost 50% were resistant to Cefazidime/ Clavulanic acid (CAC), Amoxyclav (AMC), Ciprofloxacin (CIF) and Ampicillin (AMP). The present study indicated that the effluent of fish processing industry was heavily contaminated with E.coli, S. aureus and Fecal Streptococci which confirmed improper treatment of fish processing effluent. Moreover, the precedence of antibiotic resistant E.coli may pose threat to public health safety.
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    Levels of escherichia in seafood in domestic trade and their antibiotic resistance pattern
    (Society of Fisheries Technologis(India), 2005) Surendra Raj, A.; Nirmala Thampuran; Surendran, P.K.
    Bacteriological quality of fish/shellfish sold in retail outlets in and around Cochin, India was studied. The TPC for the different samples varied from 5.5 to 8.5 log cfu/g. The total Enterobacteriaceae count varied from 2.5 to 6.5 log cfu/g. The lactose fermenters count and total coliforms count varied from 2 to 6 log cfu/g. Seafood samples had Escherichia coli count ranging from 2 to 5.5 log cfu/g. The faecal streptococci count was between 2 to 5 log cfu/ g. The majority of the E.coli isolates showed a Multiple Antibiotic Resistance (MAR) index of more than 0.5, suggesting that they may be from high risk sources. Only a low percentage of samples were acceptable for human consumption as per Indian Standards for market sold fresh fishes. Higher count of microbial indicator organisms confirms possible presence of bacteria of public health significance and demands stringent improvement in handling and sanitary practices in the markets.