FT Vol.42(2)
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- ItemStudies on Salmonella Isolates from Fishery Products(Society of Fisheries Technologists (India)Cochin, 2005)Salmonella isolates obtained from fishery products were studied with respect to their sensitivity to antibiotics, ionizing radiation and chlorine water treatment. The optimum pH range for the growth of the isolates was determined. The isolates were also characterized for pathogenicity related genes based on PCR assay. None of the 34 isolates under study were resistant to 16 antibiotics tested. All isolates were sensitive to chlorine water treatment (25 ppm) when treated in saline, but they were resistant when treatment was in organic medium. Optimum growth of all isolates was observed in the pH range of 5 to 7; however, S. typhimurium and S. worthington could grow even at pH 4. The PCR based identification of pathogenicity related genes invA and spvC showed that both genes were present in S. gallinamm and S. enteritidis and only invA was present in S. typhimurium and S. worthington. D 10 value for these serovars irradiated in brain heart infusion broth was in the range of 270 to 289 Gy.
- ItemSocio-Economic Profile of Shrimp Farmers and its Influence on the Extent of Adoption of Shrimp Culture Technologies(Society of Fisheries Technologists (India)Cochin, 2005)The study of the socio-economic profile of shrimp farmers of Nellore in Andhra Pradesh and Nagapattinam in Tamil Nadu revealed that 40% of the shrimp farmers had collegiate level of education, and they had medium levels of farming experience, information seeking behaviour, extension contact, economic motivation and risk orientation. The results of correlation analysis revealed that of the seventeen independent variables studied eleven independent variables had positive and significant relationship with the extent of adoption of shrimp culture technologies by shrimp farmers of Nellore. Among the shrimp farmers of Nagapattinam, seven independent variables had positive and significant relationship with the extent of adoption. The results of step wise multiple regression analysis revealed that variables such as information seeking behaviour, credit orientation and material possession explained 48% of the variation in the extent of adoption by shrimp farmers of Nellore and variables like extension contact, risk orientation, farming experience, type of ownership, annual income and material possession explained 84 percent of the variation in the extent of adoption by shrimp farmers of Nagapattinam
- ItemPathogenicity, Antibiogram and Biochemical Characteristics of Luminescent Vibrio harveyi, Associated with 'Black Shell Disease' of Penaeus monodon(Society of Fisheries Technologists (India)Cochin, 2005)Bacterial isolation was made from shrimps Penaeus monodon showing characteristic external symptoms of 'black shell disease' from shrimp farms located in southeast coast of India. The isolates were screened on TCBS agar plates and 0/129 for the selective isolation of Vibrio sp. Based on the morphological, biochemical and physiological characteristics, the isolate (RJM5) was characterized as luminescent Vibrio fwrveyi. Antibiogram of V. harveyi indicated that it was highly sensitive to chloramphenicol, ciprofloxacin, nalidixic acid and streptomycin. The pathogenicity studies confirmed that the isolate (V. liarveyi) was moderately virulent
- ItemImpact of Bottom Trawling on Sediment Texture and Organic Matter Along Inshore Waters of Kerala (South India)(Society of Fisheries Technologists (India)Cochin, 2005)Sediment texture and organic matter were analysed before and after trawling in samples collected from 0-50 m depth zone during December 2000-November 2001, along Kerala coast (Long. 75° 56/00 to 76o10' and Lat. 9058' to 10010'). Up to 40 m depth the composition of sand, silt and clay were 0.19 - 15.8%, 35.33 -60.53% and 29.9 - 53.12% respectively. Beyond ' this, sand was dominant (sand 32.6 - 96.15, silt 1.8 - 33.8, clay 2.45- 26.48%). In samples collected before trawling, sand, silt and clay ranged from 4.76 - 87, 45.85-55.71 and 43.18 - 47.38% respectively. In the samples collected after trawling the corresponding values for sand, silt and clay were 21.25 - 35.7, 51.28 -63.92 and 13.03 - 21.6% respectively. Organic carbon in 0-30 m depth zone ranged from 0.229 - 7.68% and it showed highest variations in July especially at depths 0-10 m and 10-20 m with respective values of 4.45 and 4.75% before trawling; while in the after trawling samples it was reduced to 2.21 % and 1.90% respectively. The sand and silt fractions showed a significant increase whereas a drastic reduction in clay fraction was observed especially in 0- 40 m depth zone due to bottom trawling
- ItemFrequency and Expression of Antibiotic Resistance in Luminous Vibrio harveyi(Society of Fisheries Technologists (India)Cochin, 2005)In-vitro activities of chloramphenicoi, ciprofloxacin, nalidixic acid and oxytetra-cycline were evaluated against penaeid shrimp larval pathogen, luminous Vibrio liarveyi. The frequency at which three strains of luminous V. harveyi strains can mutate to develop resistance to these antibiotics was also estimated. Resistant mutants developed at lower frequencies to chloramphenicol (<9.43 x lO"10) and ciprofloxacin (<1.14 x lO"10 - 1.61 x lO"9) than to nalidixic acid (6.61 x lO"5 - 8.87 x lO"5) and oxytetracycline (6.82 x lO"6 - 4.74 x lO"4) at 10 times the respective minimal inhibitory concentration (MIC). Low concentration of antibiotics (5 x MIC and 2 x MIC), however, induced more of mutant strains. Although ciprofloxacin was effective in terms of MIC and induction of low level of mutants, chloramphenicol was more effective in its ability to kill both resistant and sensitive strains of luminous V. harveyi