Detection of ctx gene positive non-O1/non-O139 V. CCholerae in shrimp aquaculture environments
Detection of ctx gene positive non-O1/non-O139 V. CCholerae in shrimp aquaculture environments
Date
2013
Authors
Rao, B.M.
Surendran, P.K.
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Water and post-larvae samples from black tiger
(Penaeus monodon) shrimp hatcheries; pond water, pond
sediment and shrimp from aquaculture farms were screened
for the presence of V cholerae. A V cholerae-duplex PCR
method was developed by utilizing V cholerae species
specific sodB primers and ctxAB genes specific primers.
Incidence of V cholerae was not observed in shrimp
hatchery samples but was noticed in aquaculture samples.
The incidence of V cholerae was higher in pond water
(7.6%) than in pond sediment (5.2%). Shrimp head (3.6%)
portion had relatively higher incidence than shrimp muscle
(1.6%). All the V cholerae isolates (n=42) belonged to non-
01/non-0139 serogroup, of which 7% of the V cholerae
isolates were potentially cholera-toxigenic (ctx positive). All
the ctx positive V cholerae (n=3) were isolated from the
pond water. Since, cholera toxin (CT) is the major
contributing factor for cholera gravis, it is proposed that
the mere presence of non-01/non-0139 V cholerae need not
be the biohazard criterion in cultured black tiger shrimp but
only the presence of ctx carrying non-01/non-0139 V
cholerae may be considered as potential public health risk.
Description
Keywords
Vibrio cholerae, Choleratoxigenic, ctx, Non-01 and non-0139, Duplex PCR, Black tiger shrimp, Penaeus monodon
Citation
Journal of Food Scienceand Technology 2013: 50(3), 496-504