Detection of ctx gene positive non-O1/non-O139 V. CCholerae in shrimp aquaculture environments

Simple item page
dc.contributor.authorRao, B.M.
dc.contributor.authorSurendran, P.K.
dc.date.accessioned2015-10-20T07:23:28Z
dc.date.available2015-10-20T07:23:28Z
dc.date.issued2013
dc.description.abstractWater and post-larvae samples from black tiger (Penaeus monodon) shrimp hatcheries; pond water, pond sediment and shrimp from aquaculture farms were screened for the presence of V cholerae. A V cholerae-duplex PCR method was developed by utilizing V cholerae species specific sodB primers and ctxAB genes specific primers. Incidence of V cholerae was not observed in shrimp hatchery samples but was noticed in aquaculture samples. The incidence of V cholerae was higher in pond water (7.6%) than in pond sediment (5.2%). Shrimp head (3.6%) portion had relatively higher incidence than shrimp muscle (1.6%). All the V cholerae isolates (n=42) belonged to non- 01/non-0139 serogroup, of which 7% of the V cholerae isolates were potentially cholera-toxigenic (ctx positive). All the ctx positive V cholerae (n=3) were isolated from the pond water. Since, cholera toxin (CT) is the major contributing factor for cholera gravis, it is proposed that the mere presence of non-01/non-0139 V cholerae need not be the biohazard criterion in cultured black tiger shrimp but only the presence of ctx carrying non-01/non-0139 V cholerae may be considered as potential public health risk.en_US
dc.identifier.citationJournal of Food Scienceand Technology 2013: 50(3), 496-504en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2061
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.subjectVibrio choleraeen_US
dc.subjectCholeratoxigenicen_US
dc.subjectctxen_US
dc.subjectNon-01 and non-0139en_US
dc.subjectDuplex PCRen_US
dc.subjectBlack tiger shrimpen_US
dc.subjectPenaeus monodonen_US
dc.titleDetection of ctx gene positive non-O1/non-O139 V. CCholerae in shrimp aquaculture environmentsen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Detection of ctx gene positive non-01non-0139.pdf
Size:
8.1 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Peer Reviewed Journal Articles (National) (M,F&B)