Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish(Pangasius hypophthalmus)
Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish(Pangasius hypophthalmus)
Date
2018
Authors
Vijayan, D.K.
Sreerekha, P.R.
Tejpal, C.S.
Asha, K.K.
Mathew, S.
Ravishankar, C.N.
Anandan, R.
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Abstract
The aim of the present study was to extract high pure acid soluble collagen (ASC) from the airbladder of
striped cat fish (Pangasius hypophthalmus). ASC was extracted and its physico-chemical characterization
was carried out to confirm the purity and structural integrity of extracted collagen. The yield of ASC
extract was determined to be 73.4±0.8% of the dry weight of raw material. Amino acid analysis revealed
the glycine content as 243.0±1.3 residues/1000 residues, imino acids as 186.1±1.4 residues/1000 residues
respectively and was shown to be devoid of non-collagenous amino acids such as tryptophan.
Electrophoretic analysis confirmed the subunit pattern of type I collagen and showed that it also
contained β and ɤ subunits. UV/Vis absorption and FT-IR analysis demonstrated the primary and
secondary structural integrity of collagen and also indicated that no denaturation had occurred during
extraction process. 1H-NMR analysis validated the structural integrity of collagen triple helix. The
denaturation temperature (Td) of ASC was assessed as 33.3 °C. The air bladder collagen extracted from
striped cat fish is of high purity and belongs to type I with intact triple helical structure
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Citation
Int. J. Fish. Aquat. Stud. 6(4):310-318