Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish(Pangasius hypophthalmus)
Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish(Pangasius hypophthalmus)
| dc.contributor.author | Vijayan, D.K. | |
| dc.contributor.author | Sreerekha, P.R. | |
| dc.contributor.author | Tejpal, C.S. | |
| dc.contributor.author | Asha, K.K. | |
| dc.contributor.author | Mathew, S. | |
| dc.contributor.author | Ravishankar, C.N. | |
| dc.contributor.author | Anandan, R. | |
| dc.date.accessioned | 2019-08-23T09:59:43Z | |
| dc.date.available | 2019-08-23T09:59:43Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | The aim of the present study was to extract high pure acid soluble collagen (ASC) from the airbladder of striped cat fish (Pangasius hypophthalmus). ASC was extracted and its physico-chemical characterization was carried out to confirm the purity and structural integrity of extracted collagen. The yield of ASC extract was determined to be 73.4±0.8% of the dry weight of raw material. Amino acid analysis revealed the glycine content as 243.0±1.3 residues/1000 residues, imino acids as 186.1±1.4 residues/1000 residues respectively and was shown to be devoid of non-collagenous amino acids such as tryptophan. Electrophoretic analysis confirmed the subunit pattern of type I collagen and showed that it also contained β and ɤ subunits. UV/Vis absorption and FT-IR analysis demonstrated the primary and secondary structural integrity of collagen and also indicated that no denaturation had occurred during extraction process. 1H-NMR analysis validated the structural integrity of collagen triple helix. The denaturation temperature (Td) of ASC was assessed as 33.3 °C. The air bladder collagen extracted from striped cat fish is of high purity and belongs to type I with intact triple helical structure | en_US |
| dc.identifier.citation | Int. J. Fish. Aquat. Stud. 6(4):310-318 | en_US |
| dc.identifier.uri | http://drs.cift.res.in/handle/123456789/4201 | |
| dc.language.iso | en | en_US |
| dc.title | Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish(Pangasius hypophthalmus) | en_US |
| dc.type | Article | en_US |