Collagen Biosynthesis in Osteoblast Cells Treated with Fish Collagen Peptides
Collagen Biosynthesis in Osteoblast Cells Treated with Fish Collagen Peptides
Date
2017
Authors
Hema, G.S
Shyni, K
Prasad, M. Manu
Ninan, George
Mathew, Suseela
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India),COCHIN
Abstract
Collagen is one of the most widely used biomaterials
in tissue engineering and regenerative medicine. To
investigate the effect of fish collagen peptides on the
formation of collagen proteins by mature osteoblast
cells, human osteoblast cells were treated with
collagen peptide in both time and dose dependent
manner. The stimulatory effect of collagen peptide
was confirmed by western blot and immunocytochemistry
analysis. The results clearly indicate that
the presence of fish collagen peptides led to a dose
dependent increase in collagen synthesis by osteoblast
cells upto a concentration of 0.6 mg ml-1.
However native collagen or collagen free protein
hydrolysate failed to stimulate the production of
collagen in osteoblast cells. The results show a
stimulatory effect of fish collagen peptides on the
collagen biosynthesis in osteoblast cells and suggest
a possible mechanism for the regulation of collagen
turnover in joint tissues.
Description
Keywords
Fish collagen peptides, osteoblast cells, collagen quantification, western blotting, immunocytochemistry
Citation
Fishery Tech. 54(2) :107-111