Tissue proteinase activity in structural proteins of mackerel
Tissue proteinase activity in structural proteins of mackerel
Date
1998
Authors
Jose, L.
Raghunath, M.R.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists(India),Cochin
Abstract
Tissue proteinase activity (TPA) in mackerel (Rastrelliger kanagurta) was studied. Extraction of sarcoplasmic fraction from muslce did not completely remove TPA from myofibrillar fraction. With inclusion of sodium dodecyl sulphate it was possible to extract more protein into the sarcoplasmic fraction, but TPA was still present in the myofibrillar fraction, though to a lesser extent, in both autolytic and proteolytic modes of assay. Repeated extraction of muscle with low ionic strength buffers could not completely free the myofibrillar fraction from TPA indicating its strong attachment to this fraction.
Description
Keywords
Mackerel, tissue proteinase activity, structural proteins
Citation
Proceedings of the Symposium on "Advances and Priorities in Fisheries Technology" held during 11-13 Feb, 1998, Cochin ed. by Balachandran, K.K. et. al, 322-327