Multiresidue analysis of multiclass pesticides and polyaromatic hydrocarbons in fatty fish by gas chromatography tandem mass spectrometry and evaluation of matrix effect
Multiresidue analysis of multiclass pesticides and polyaromatic hydrocarbons in fatty fish by gas chromatography tandem mass spectrometry and evaluation of matrix effect
Date
2016
Authors
S. Chatterjee, Niladri
Utture, Sagar
Banerjee, Kaushik
Ahammed Shabeer, T.P.
Kamble, Narayan
Mathew, Suseela
Ashok Kumar, K.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
This paper reports a selective and sensitive method for multiresidue determination of 119 chemical residues
including pesticides and polyaromatic hydrocarbons (PAH) in high fatty fish matrix. The novel sample
preparation method involved extraction of the target analytes from homogenized fish meat (5 g) in
acetonitrile (15 mL, 1% acetic acid) after three-phase partitioning with hexane (2 mL) and the remaining
aqueous layer. An aliquot (1.5 mL) of the acetonitrile layer was aspirated and subjected to two-stage dispersive
solid phase extraction (dSPE) cleanup and the residues were finally estimated by gas chromatography
mass spectrometry with selected reaction monitoring (GC–MS/MS). The co-eluted matrix
components were identified on the basis of their accurate mass by GC with quadrupole time of flight
MS. Addition of hexane during extraction and optimized dSPE cleanup significantly minimized the matrix
effects. Recoveries at 10, 25 and 50 lg/kg were within 60–120% with associated precision, RSD < 11%.
Description
Keywords
Fatty fish, Pesticide residues, Polyaromatic hydrocarbons, Sample preparation, Gas chromatography mass spectrometry, Matrix effect
Citation
Food Chemistry 196 : 1–8 , http://dx.doi.org/10.1016/j.foodchem.2015.09.014