Analysis of the matrix protein gene sequence of the Asian lineage of peste-des-petits ruminants vaccine virus
Analysis of the matrix protein gene sequence of the Asian lineage of peste-des-petits ruminants vaccine virus
Date
2006
Authors
Muthuchelvan, D.
Sanyal, A.
Sreenivasa, B.P.
Saravanan, P.
Dhar, P.
Singh, R.P.
Bandyopadhyay, S.K.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The M gene nucleotide sequence of an Indian peste-des-petits ruminants (PPRV) vaccine virus (‘‘PPRV Sungri/96’’) belonging to Asian lineage was determined. The gene is 1476 nucleotides long with a single open reading frame (ORF). The nucleotide and predicted amino acid sequence was compared with the homologous region of the African Lineage Vaccine virus ‘‘PPRV/Nigeria/75/1’’. The nucleotide sequence of the ‘‘PPRV Sungri/96’’ was 86% identical to that of ‘‘PPRV/Nigeria/75/1’’, while a homology of 93% and 95% could be observed in the ORF and amino acids level, respectively. The M gene encodes a protein of 335 amino acids, with a predicted molecular weight (MW) of 37.8 kDa. The ORF is flanked by a 30 untranslated region of 436 nucleotides and a high level of sequence divergence ( 30%) could be observed in this region between the vaccine viruses of Asian and African lineages. A high degree of conservation of several amino acids of this protein observed previously was also
confirmed in this study.
Description
Keywords
Morbillivirus, peste-des-petits ruminants virus, vaccine virus, m gene, nucleotide sequence, amino acid sequence
Citation
Veterinary Microbiology 2006: 113(1), 83-87