Journal articles (MFB)

Permanent URI for this collection

http://localhost:4000/handle/123456789/121

Browse

Browsing Journal articles (MFB) by Author "Das, S."

Now showing 1 - 3 of 3
  • Item
    Detection and differentiation of listeria monocytogenes and listeria innocua by multiplex PCR
    (Society of Fisheries Technologists(India),Cochin, 2010) Das, S.; Surendran, P.K.; Thampuran, N.
    The present study has been designed to differentiate L. monocytogenes from L. innocua in a single tube Multiplex PCR reaction.
  • Item
    Detection of listeria monocytogenes from freshwater fish, prawn and chicken meat by direct nested PCR
    (Asian Fisheries Society, 2011) Das, S.; Singh, V.P.; Ltu, K.; Kathiresan, S.; Sharma, B.; Bhilegaonkar, K.N.
    Three different methods of processing samples from freshwater fish, prawn and chicken meat were compared with nested PCR for the detection of Listeria monocytogenes. Nested PCR failed to detect the organism from freshwater fish, prawn and chicken meat when processing was done by boiling lysis method, but it could detect the organisms from freshwater fish, prawn and chicken meat up to the spiking level of 104, 103 and 105 cfu.g-1, respectively while processing of sample was done by phenol extraction without enrichment. However, using phenol extraction after enrichment, the sensitivity was found higher than without enrichment and it was possible to detect up to the spiking level of 102, 10 and 103 cfu.g-1 in case of fish, prawn and chicken meat, respectively.
  • Item
    Isolation and molecular characterisation of atypical enterotoxigenic bacillus cereus with negative voges-proskauer reaction from Indian white shrimp fenneropenaeus indicus (H. Milne Edwards, 1837)
    (ICAR, 2013) Das, S.; Lalitha, K.V.; Thampuran, N.
    Enterotoxigenic Bacillus cereus is one of the important pathogenic organisms, which causes two distinct type of food poisoning in human beings viz., emetic type and diarrhoeal type. As per biochemical tests, B. cereus is generally Voges-Proskauer (VP) positive. In the present study, two enterotoxigenic B. cereus isolates from white shrimp (Fenneropenaeus indicus) with negative VP reaction have been reported. Both the isolates were confirmed as B. cereus by other biochemical tests as well as molecular methods like PCR and 16S rRNA gene sequencing. They were also found to produce diarrhoeal enterotoxin by reverse passive latex agglutination (RPLA) test. The enterotoxin producing genes, hbla and entb were detected in both the isolates