Biochemistry & Nutrition

Permanent URI for this community

http://localhost:4000/handle/123456789/51

Browse

Now showing 1 - 20 of 308

Amino Acid Composition of 27 Food Fishes and Their Importance in Clinical Nutrition
(Hindawi Publishing Corporation, 2014) Mohanty, B.; Mahanty, A.; Ganguly, S.; Sankar, T.V.; Chakraborty, K.; Anandan, R.; Paul, B.N.; Sarma, D.; Mathew, S.; Asha, K.K.; Behera, B.K.; Aftabuddin, Md.; Debnath, D.; Vijayagopal, P.; Sridhar, N.; Akhtar, M.S.; Sahi, N.; Mitra, T.; Banerjee, S.; Paria, P.; Das, D.; Das, Pushpita; Vijayan, K.K.; Laxmanan, K.K.; Sharma, A.P.
Proteins and amino acids are important biomolecules which regulate key metabolic pathways and serve as precursors for synthesis of biologically important substances; moreover, amino acids are building blocks of proteins. Fish is an important dietary source of quality animal proteins and amino acids and plays important role in human nutrition. In the present investigation, crude protein content and amino acid compositions of important food fishes from different habitats have been studied. Crude protein content was determined by Kjeldahl method and amino acid composition was analyzed by high performance liquid chromatography and information on 27 food fishes was generated. The analysis showed that the cold water species are rich in lysine and aspartic acid, marine fishes in leucine, small indigenous fishes in histidine, and the carps and catfishes in glutamic acid and glycine.The enriched nutrition knowledge base would enhance the utility of fish as a source of quality animal proteins and amino acids and aid in their inclusion in dietary counseling and patient guidance for specific nutritional needs.
Amphiphilic dendrimer as reverse micelle: synthesis, characterization and application as homogeneous organocatalyst
(Elsevier, 2019) Mole, P.B.S.; George, S.; Shebitha, A.M.; Kannan, V.; Mathew, S.; Asha, K.K.; Sreekumar, K.
The core and surface terminal groups are the two main catalytic sites in a dendrimer. In most of the reported examples, the catalytic sites in dendritic catalysis are the surface terminal functional groups. This perspective article concerned with the dendrimer based catalysis, involving these two catalytic sites and the dendrimer cavities. The interior cavities provide the nanoscale reactor sites, by creating reverse micelle like appearance for catalysis. In exploring the significant achievements in this area, a low generation PAMAM dendrimer with amphiphilic nature, having a polymeric core with large number of pendent amino groups was synthesized and concentrated its catalytic activity. The key features with respect to positive and/or negative catalytic activity was highlighted by synthesizing various aryl and heteroaryl 2-substituted benzimidazoles. The synthesized dendritic organocatalyst was proved to be amazingly reactive and gave high yield of products within a few minutes at room temperature with low catalyst loading. Here, a new stable hemiaminal, the species rarely been detected and much less isolated in bulk, was obtained during the synthesis of benzimidazoles. Moreover, this is the first reported method for the synthesis of benzimidazoles, using the homogeneous PAMAM dendrimer as a basic organocatalyst
ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF LIVER OILS OF FOUR SHARK SPECIES FROM INDIAN EEZ
(WILEY, 2008) MATHEW, MATHEN; MATHEW, SUSEELA; NAIR ASHOK KUMAR, KESAVAN; ANANDAN, RANGASAMY
The analgesic and anti-inflammatory properties of liver oils of four different sharks, namely Neohariotta raleighana, Centrosymnus crepidater, Apristurus indicus and Centrophorus scalpratus, captured from the Arabian Sea and the Indian Ocean were evaluated. While the analgesic property was determined using the acetic acid-induced mouse writhings and hot-plate reaction time, the anti-inflammatory activity was evaluated using the formalin-induced rat-paw edema. The oils examined were found to possess significant (P < 0.05) analgesic activity against acetic acid-induced writhings and hot-plate reaction in mice. In the formalin-induced edema, a significant (P < 0.05) inhibition of inflammation was observed between the 2nd and 4th hour showing 58–65% inhibition. These results suggest that liver oils of sharks from Indian waters are effective as analgesic and anti-inflammatory agents. The role of lipid components (squalene, alkylglycerols and polyunsaturated fatty acids) on anti-inflammatory and antinociceptive properties is highlighted. Inhibition of the synthesis of prostaglandins and other inflammatory mediators which probably account for the properties is discussed.
Animal experiments on nutrition
(ICAR - Central Institute of Fisheries Technology, Cochin, 2012) Ganesan, B.
Anti diabetic and anti hyperlipidemic activities of different extracts of ailanthus malabarica stem bark in alloxan induced diabetic rats
(Pharma Intelligence, 2015) Louis, T.; Yuvaraj, P.; Madhavachandran, V.; Anandan, R.
To evaluate the anti diabetic and anti hyperlipidemic activities of different extracts of Ailanthus malabarica stem bark in alloxan induced diabetic rats. Different extracts (hexane, ethanol and water, 50 mg/kg) of A. malabarica stem bark were administered to alloxan-induced diabetic rats for 21 days and blood glucose levels of the diabetic rats were monitored at one week intervals. Lipid profiles of the treated diabetic rats were determined after the period of treatment. Treatment of alloxan-induced diabetic rats with ethanol extract of A. malabarica stem bark caused a significant (P<0.05) reduction in fasting blood glucose levels of the diabetic rats in 21 days treatment. The ethanol extract showed a comparable action with the reference drug, glibenclamide. The ethanol extract exerted a significant reduction in the levels of serum total cholesterol, triglycerides, LDL, VLDL and phospholipids, and increase in HDL levels of the diabetic rats. However, water and hexane extract of A. malabarica stem bark did not show any such beneficial effects. . These results suggest that the ethanol extract of A. malabarica stem bark possesses anti diabetic effect on alloxan induced diabetic rats and this justifies its usage in ethno medicine and can be exploited in the management of diabetes.
Anti-viral properties of carrageenan, a sulphated polysaccharide derived from marine red algae against Covid 19 (SARS-CoV-2) infection- Future prospective
(IJSEAS, 2021) P, Amruth; P., Akshay; Jacob M., Rosemol; Anandan, R.; Mathew, Suseela
The novel coronavirus (nCoV) disease COVID-19, triggered by SARS-CoV-2 is a major threat caused to the public health in the 21th century. Combating the same is a herculean challenge for the scientific world. This is represented as the third corona virus outbreak in preceding 20 years, followed by SARS (2002- 2003) and MERS (2012). However, there is no clinically effective drugs against COVID-19 virus infection. For International medical community, development of effective drugs to avoid and cure a viral attack is an urgent requisite during this time. As a consequence, we have piloted an organised review presenting the anti-viral prospective of carrageenan, a sulphated galactan derived from red seaweed. Sulphated polysaccharides are demonstrated as potential antiviral that can intrude the initial stages of viral infections, have wide prospects in anti-corona virus applications. Carrageenan and its oligosaccharides are well reported for its anti-viral properties; chiefly via the obstruction of the attachment stage of virus. The current review presents the survey of antiviral prospective of carrageenan and aims to provide new approach to researchers for exploring seaweed polysaccharides to develop effective therapy against strains of SARS-CoV-2 virus.
Antimicrobial activity of seaweed extracts of Padina gymnospora extracted by supercritical and conventional method
(ICAR - Central Institute of Fisheries Technology, Cochin, 2019) Anupama, T.K.; Kumar, L.R.G.; Mathew, S.
Antioxidant and hepatoprotective property of squalene for counteracting the oxidative damage induced by methotrexate in experimental rats
(Acta Biologica Szegediensis, 2020) Sumi, Edakkukaran Sudhakaran; Dara, Pavan Kumar; Mannuthy, Rosemol Jacob; Ganesan, Balaraman; Anandan, Rangasamy; Mathew, Suseela
Methotrexate (MTX), an antifolate drug, is extensively prescribed for patients suff ering from diseases like cancer, psoriasis, neoplasms, and rheumatoid arthritis. Despite its eff ectiveness, MTX sometimes fi nds limited application because its undesirable side eff ects, including hepatic or renal impairment, bone marrow toxicity and gastrointestinal mucosal injury. Squalene, a highly unsaturated isoprenoid compound, isolated from shark liver oil has great potential in neutralizing the damaging eff ects triggered by free radicals. Therefore, in this study, the protective role of dietary squalene supplementation on oxidative stress induced by methotrexate in experimental rats was evaluated. A signifi cant reduction was displayed in the activities of catalase (CAT) and superoxide dismutase (SOD) in MTX-intoxicated groups compared to other groups. Similarly, the activities of glutathione dependant enzymes (GPx and GST) and reduced glutathione (GSH) in MTX-induced groups were shown to be lower compared to the untreated control. Increased LPO (lipid peroxide) level was found in MTX-intoxicated groups compared to other groups. In addition, alterations in the levels of liver marker enzymes like AST, ALP, ALT, and LDH were noticed in MTX intoxicated groups compared to other groups. Biochemical results were confi rmed by the histopathological examination of liver sections. In conclusion, the result obtained in the present study proposes that squalene exerts antioxidant activity and is capable of ameliorating oxidative stress and liver injury induced by MTX.
Antioxidant defense of betaine against isoprenaline-induced myocardial infarction in rats
(Springer-Verlag, 2010) Ganesan, B.; Buddhan, S.; Anandan, R.; Sivakumar, R.; Anbinezhilan, R.
We investigated the antioxidant preventive effect of betaine on isoprenaline-induced myocardial infarction in male albino rats. Isoprenaline induced myocardial infarction was manifested by a moderate elevation in the levels of diagnostic marker enzymes (alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and creatine phosphokinase) and homocysteine in plasma of experimental rats. Significant rise in the level of lipid peroxidation with a concomitant decline in the levels of myocardial non-enzymic (reduced glutathione) and enzymic antioxidants (glutathione peroxidase, glutathione-S-transferase, catalase and superoxide dismutase) was also observed. Oral pretreatment with betaine significantly prevented isoprenaline-induced alterations in the levels of diagnostic marker enzymes and homocysteine in plasma of experimental groups of rats. It counteracted the isoprenaline-induced lipid peroxidation and maintained the myocardial antioxidant defense system at near normal. Histopathological observations also confirmed the protective effect of betaine against isoprenaline-induced myocardial infarction. The results of the present investigation indicate that the protective effect of betaine is probably related to its ability to strengthen the myocardial membrane by its membrane stabilizing action or to a counteraction of free radicals by its antioxidant property.
Antioxidant defense of fish collagen peptides attenuates oxidative stress in gastric mucosa of experimentally ulcer-induced rats
(Springer, 2021) K Vijayan, Divya; Sreerekha, P R; Kumar Dara, Pavan; Ganesan, B; Mathew, Suseela; Anandan, Rangasamy; Ravisankar, C N
The aim of the present study was to investigate the ability of fish collagen peptides (FCP) from the skin of great hammerhead shark (Sphyrna mokarran) to avert the occurrence of gastric ulcer in experimental rats. FCP treatment prevented the formation of ulcerative lesions on gastric tissues with 86% of inhibition. The histopathology analysis of gastric tissue revealed that the FCP intake prevented the occurrence of hemorrhage and erosion in gastric tissue with formation of mild edema and necrosis, as well as normalized the pH and volume of gastric juice. It also downregulated the expression of pro-inflammatory marker interferon-ɤ (IFN-ɤ) and upregulated the anti-inflammatory marker interleukin-4 (IL-4) in gastric tissue. FCP is capable to modulate the oxidative stress by enhancing the activity of antioxidant defense enzymes superoxide dismutase (SOD) and catalase and by lowering the levels of membrane lipid peroxidation.
Antioxidant potential of water hyacinth (eichornia crassipes): In vitro antioxidant activity and phenolic composition
(Taylor & Francis, 2013) Surendraraj, A.; Farvin, K.H.S.; Anandan, R.
The aims of the present study were (a) to extract and quantify the main phenolic acids and tocopherols from the petiole, leaves, and flowers of Eichornia crassipes; (b) to evaluate the antioxidant capacity of the extracts in four in vitro systems (1,1-diphenyl-2-pycryl-hydrazyl [DPPH] radical scavenging ability, iron chelating activity, reducing power, and prevention of oxidation in a liposome model system); and (c) its effectiveness in retarding lipid peroxidation in fish oil by accelerated stability test. Significant differences were observed in total and individual phenolic contents and in the antioxidant activities of extracts from the various parts of E. crassipes. Out of the 11 phenolic acids analyzed, ethanolic extracts contained high amounts of gallic, protocatechuic, gentisic, and p-hydroxybenzoic acid, whereas, water extracts contained less amounts of a varied number of phenolic acids. Ethanolic extracts of flower, which contained the highest total phenolic content, were found to have high DPPH radical scavenging activity and reducing power. However, ethanolic extracts of leaf exerted a high Fe2+ chelating activity and also inhibited lipid peroxidation process both in liposomes and fish oil. Our results demonstrate that E. crassipes, an underutilized aquatic weed, could be a potential natural antioxidant source for food, feed, and pharmaceutical applications.
Antioxidant, functional properties and amino acid composition of pepsin-derived protein hydrolysates from whole tilapia waste as influenced by pre-processing ice storage
(2017) Tejpal, C.S.; Vijayagopal, P.; Elavarasan, K.; Linga Prabhu, D.; Lekshmi, R.G.K.; Asha, K.K.; Anandan, R.; Chatterjee, N.S.; Mathew, S.
Application of collagen and collagen peptides in human nutrition: a review
(ICAR - Central Institute of Fisheries Technology, Cochin, 2016) Vijayan, D.K.; Sreerekha, P.R.; Jayarani, R.; Vimaladevi, S.; Navaneethan, R.; Chatterjee, N.S.; Mathew, S.; Mohanty, B.P.; Anandan, R.
Aquaceuticals for developing high value noble foods and dietary supplements
(2017) Suseela Mathew; Tejpal, C.S.; Lekshmi, R.G.K.; Zynudheen, A.A.; Ravishankar, C.N.
Assessment of Efficiency of an Indigenous Liquid Smoke for masmin Production
(Society of Fisheries Technologists(India), 2016) Nithin, C.T.; Yathavamoorthi, R.; Niladhri, S.C.; Ananthanarayanan, T.R.; Suseela, M.; Bindu, J.; Gopal, T.K.S.
The aim of this study was to develop a methodology for the production of liquid smoke from coconut husk and to evaluate efficiency of the same in masmin production. The developed method can produce 1.5 l of liquid smoke per hour. It had total phenolic content of 1348 ppm and carbonyl content of 38.03 mg ml-1. Titratable acidity and pH were found to be 1.88% by weight of acetic acid and 4.34, respectively. Total PAH content in the liquid smoke was found to be 0.79 ppb of which phenanthrene was found in highest level (0.54 ppb). Benzo[a]pyrene was not detected. The developed liquid smoke was found to be compliant with current regulatory limits in terms of PAH. It was observed that soaking of cooked skipjack tuna loins in liquid smoke added with 5% salt, for a duration of 30 min produced masmin with similar flavour of traditional masmin.
Assessment of polyaromatic hydrocarbons and pesticide residues in domestic and imported pangasius (Pangasianodon hypophthalmus) fish in India
(Society of Chemical Industry, 2015) Chatterjee, N.S.; Banerjee, K.; Utture, S.; Kamble, N.; Madhusudana Rao, B.; Panda, S.K.; Suseela Mathew
The pangasius catfish (Pangasianodon hypophthalmus) is a ubiquitous item of seafood in global markets. However, pesticide residues in aquaculture fish, arising from agricultural run-off and/or direct application during pond preparation, pest control and harvest, are a potential food safety concern. This study assessed the level of chemical hazard in domestic and imported pangasius fish in India. A total of 119 contaminants, including polyaromatic hydrocarbons (PAHs), organochlorines (OCs) and other groups of pesticides, were screened in 148 samples during 2014–2015 as the first endeavour of its kind in an Indian context, employing a validated gas chromatography/tandem mass spectrometry (GC/MS/MS) method. Apart from the low-level incidence of OCs, pesticides such as quinalphos, malathion, parathion-methyl, etc.weredetected in38%of the samples. Incomparisonwith Indian pangasius, fewer contaminants at low residue level were detected in pangasius fillets imported from Vietnam. The human dietary exposures of the residue concentrations detected were less than the maximum permissible intakes and hence appeared safe. However, detection of commonly used pesticides indicated their direct application in aquaculture and contamination from agricultural run-off. This emphasizes the need for continual residue monitoring in aquaculture fish.
Authentication of two bio-active fish oils by qualitative lipid profiling using semi-targeted approach: an exploratory study
(2020) Chatterjee, N.S.; Singh, A.; Vishnu, K.V.; Ajeeshkumar, K.K.; Anandan, R.; Kumar, K.A.; Mathew, S.
Authentication of Two Bio-Active Fish Oils by Qualitative Lipid Profiling Using Semi-Targeted Approach: An Exploratory Study
(Journal of AOAC International, 2020) S. Chatterjee, Niladri; Singh, Akanksha; Vishnu, K .V.; Ajeeshkumar, K.K.; Anandan, R.; Ashok Kumar, K.; Mathew, Suseela
Background: Fish oils, which are rich in health-promoting polyunsaturated fatty acids (PUFA), have emerged as promising functional foods in the global health and wellness food market. Their source regarding the fish type, season, and location of harvesting might influence the nutritional value of such bioactive oils and determine their market price. The differences in price among such oils often lead to economically motivated mislabeling and adulteration. Objective: In this study, our objective was to demonstrate how a qualitative targeted shotgun lipid profile workflow using an electrospray ionization-quadrupole-linear ion trap MS (QTrap) could differentiate fish oils originating from two different species. Methods: Five samples each of sardine (Sardinella longiceps) oil and shark (Echinorhinus brucus) liver oil were diluted to a concentration of 80 µg/mL in chloroform-methanol (1 + 2, v/v) with 5 mM ammonium acetate. These samples were directly infused into a QTrap MS. The data were acquired for 23 precursor ion and 4 neutral loss scan experiments in the positive ionization mode and compared. Results: We identified the following major lipid classes: cholesteryl ester, diacyl glycerol, triacylglycerol, monoalkyldiacylglycerol, and phophatydyl choline. The relative peak areas of the identified lipid species, when subjected to supervised multivariate analysis, could effectively distinguish the sardine oil and shark liver oil. Conclusions: The approach will be useful in establishing authenticity of fish oil and to support the regulatory agencies in dispute resolution. It can also be extended to establish authenticity in other agricultural and food commodities. Highlights: This paper reports a proof of concept for authenticating PUFA-rich fish supplements. A shotgun targeted lipidomics profile and chemometrics modeling successfully discriminated sardine oil and shark liver oil.
Behaviour of mrigal (Cirrhinus mrigala) meat components during the washing process in the preparation of surimi
(Society of Fisheries Technologists(India), 2002) Sankar, T.V.; Ramachandran, A.
To elucidate the effect of washing, on flesh components, mrigal flesh was washed through one, two and three washing cycles. Washing resulted in absorption of water (1-3%) and loss of fat (49%). 35% loss of soluble protein (SP) was noticed in the first washing itself and the loss is almost equally shared by the sarcoplasmic (18% of SP) and the myofibrillar proteins (17% of SP). The subsequent washings removed small portions of water-soluble sarcoplasmic proteins resulting in the concentration of myofibrillar proteins. 73% of the soluble protein was retained in the flesh after three washing cycles. The protein had undergone marginal conformational changes as reflected by the decrease in the actomyosin Ca super(2+) ATPase activity The rheological properties of the washed flesh were, however, significantly better than that of the unwashed mince.
Beverage preparation from fish hydrolysates
(Society of Fisheries Technologiste (India), 1975) Prabhu, P.V.; Radhakrishnan, A.G.; Arul James, M.
A method for the preparation of energy food incorporating fish hydrolysates, sugar, cocoa, malt extract etc. is described. The product has good consumer appeal. The preparation does not impart any bitter hydrolysate. It freely mixes with hot or cold milk and the resulting drink is adjudged to be very palatable.

Browse

Browsing Biochemistry & Nutrition by Title

Results Per Page

Sort Options